Studies on Bio-color Production by Pseudomonas aeruginosa Isolated from Soil

This study aimed at producing biocolor from soil inhabiting bacteria. Soil samples were screened for isolation of green pigment-producing bacteria and identified molecularly using the standard method. The effect of medium, pH, temperature, incubation time, shaking and static conditions on color production were determined on the isolate, and the pigment was extracted by using chloroform. It was observed that green pigment was produced by Pseudomonas aeruginosa in Nutrient broth at pH 7, after 72 hours incubation and at the temperature of 37°C under shaking condition at 4,000 rpm for 15 minutes. The pigments were characterized and identified as pyocyanin using Thin Layer Chromotography (TLC), Fourier Transformed Infrared Spectroscopy (FTIR) and UV-Visible spectroscopy (UV). The stability of the pigment was tested based on pH and temperature. It was found that the green pigment showed stability at 160°C, 200°C and pH 13.