Study the morpho-physiological leaves behavior of grafted and ungrafted olive trees (Olea europaea) under saline stress

The objective of this work is to determine the effect of salinity on the morpho-physiological behavior of young olive plants in cuttings and grafted plants, including two local varieties (Chemlal and Sigoise) and two introduced varieties (Manzanilla and Arbiquina). The experiment took place in a greenhouse with a well-controlled condition. The experimental plan is in a complete randomization of two factors (salinity and varieties); plants without NaCl application were used as control. The plant material (young 2-year-old olive seedlings) was selected and brought from a crop nursery. The experimentation started by the irrigation of the control plant with a nutrient solution. About the salinity treatment, the tree seedlings received a nutrient solution fortified by the addition of 100mM of NaCl. Four repetitions are being done according to the field capacity. The study covered the variation of the relative water content in leaves as well as the leaf surface, stomata density, stomatal size and the wax rate on the upper foliar epidermis. RWC leaf area and stomatal density of the treated plants decreased compared to the control. On the other hand, the wax level increases in the case of salt stress compared to the control, both for the grafted plants and for the plants not grafted.