In Vitro Production of Rice Plants from Synthetic Seeds by Encapsulation of Somatic Embryos

Somatic embryos of rice [Oryza sativa (cv.Tellahamsa)] were encapsulated in an alginate matrix to produce synthetic seeds. Eight to ten-day-old globular somatic embryoids were found suitable for germination and plant conversion. In the present investigation, 2.5 % sodium alginate was found suitable for encapsulation of somatic embryos of rice cultivar Tellahamsa. Encapsulated somatic embryos were transferred onto MS full strength and 1/2 strength regeneration medium containing IAA, BAP, KN and MS basal media. Efficient development of shoots and roots (36%) was observed on the medium containing 1 mg/l IAA, 2mg/l BAP and 0.6 mg/l KN. Germinated synthetic seeds were transferred successfully to the net house.