Micropropagation of Pedalium murex L. Through Shoot Tip Explant Culture

In vitro regeneration was developed from shoot tip explants of Pedalium murex. MS medium supplemented with different concentration of BAP (2 mg/l - 8mg/l) and IAA (2 mg/l) respectively were tested for their efficiency multiple shoots induction. The highest rate of multiple shoots induction was observed on BAP (4 mg/l) and in combination with IAA (2 mg/l). The well developed shoots were subjected to subculture for rooting on MS medium fortified with different concentration of IBA (1mg/l- 4 mg/l). However in this experiment 3mg/l of IBA induced more roots. After 20 days rooted plantlets were transfer to the acclimatization process. The highest plant survival percentage (73%) after two month acclimatization period.