Mass Propagation of Spilanthes calva L. Through Shoot tip Explants

Mass Propagation of Spilanthes calva .L was achieved through shoot tip explants. The explants were cultured on MS medium supplemented with BAP (4.44-22.20 �M) in combination with NAA (2.69 �M ). The rate of shoot proliferation was very high at 13.32 �M BAP and 2.69 �M NAA. The regenerated shootlets were excised and transferred to MS medium containing different concentration of IBA. Induction of root was maximum at 9.80 �M IBA. All the regenerated plantlets were hardened and successfully established in the normal environment.