BIOGENIC SYNTHESIS OF Solanum trilobatum MEDIATED BY SELENIUM NANOPARTICLES AND ITS CYTOTOXICITY AND ANTIOXIDANT ACTIVITY

Aim: To estimate the antioxidant and cytotoxicity activity of Solanum trilobatum mediated by selenium nanoparticles.

Introduction: Solanum trilobatum, commonly called thuthuvalai, is one of the most important medicinal plants that grows in the southern regions of India. It is traditionally used in curing various ailments. It is said to cure certain diseases like tuberculosis, respiratory problems and bronchial asthma. Phytochemical screening of various extracts such as chloroform, water of S. trilobatum showed the presence of secondary metabolites such as steroids, triterpenoids, sugars, reducing sugars, phenolic compounds. S. trilobatum is said to possess antimicrobial activity, cytotoxicity activity, protective effect, haemolytic activity.

Materials and Methods: Plant extract was prepared, and synthesis of nanoparticles was done using UV-Beckmann spectrometer. The synthesized nanoparticles were then centrifuged. Pellet collection was done after centrifugation. This was used for testing different properties like antioxidant and cytotoxicity. The antioxidant property was tested using a DPPH assay where ascorbic acid was used as a standard solution. For the cytotoxicity activity, wells were used, where in the mortality rate of the shrimps was estimated when the plant extract mediated selenium nanoparticle of different concentration was added.

Results: Solanum trilobatum mediated by selenium nanoparticles showed an increased LD 50 in higher concentration in the case of cytotoxicity activity. There was a significant effect of the plant extract mediated selenium nanoparticle when compared with the standard ascorbic acid in the antioxidant activity.

Conclusion: Thus, Solanum trilobatum mediated selenium nanoparticle expresses good antioxidant and potent cytotoxicity activity. Hence, it can be taken up for further evaluation.