DEVELOPMENT, IMPLEMENTATION OF AN EFFICIENT AND CHEAP METHOD FOR DNA EXTRACTION FROM DRY LEAVES OF DATE PALM WITHOUT USING LIQUID NITROGEN

Date palm (Phoenix dactylifera L.) is the most significant economic fruit tree planted in Iraq. DNA isolation is one of the most important steps in the application of molecular biology, especially dependent on PCR and other techniques. The difficulty of DNA extracting from palm tissue could be because the solidity and high content of polyphenolic and polysaccharides compounds, that consequently obstruct DNA extraction and have prompte researchers to find the efficient methods for the isolation. This study describeds an effictive method for DNA extraction by using CTAB (Cetyl Trimethyl Ammonium Bromide) without liquid nitrogen. We have developed a simple and reliable method for DNA isolation from the leaves for six of date palm cultivars, the obtained results recorded significant concentration and purity of isolated DNA which, were (567.6 -1047.1) ng/µL, and (1.82-2.00) nm respectively. DNA product was tested by four SSR-PCR markers, to produce 61 clear bands, consequently, our result confirm that a modified method is perfect to DNA extraction from plant tissue without liquid nitrogen.