COMPARATIVE EFFECTIVENESS OF CHEMOTHERAPEUTICS AGENTS AGAINST Pseudomonas aeruginosa COLLECTED FROM DIFFERENT REGION’S OF KARACHI DURING 2014-2015

Background: Antibiotic resistances for Pseudomonas aeruginosa are increasing worldwide. Due to the irrational use of antibiotics, the resistance of antibiotics has been augmented which results increase in morbidity and mortality with the span of time. World renowned regulatory bodies like Food and Drug Administration (FDA), Center of Disease Control and Prevention (CDC), and World Health Organization (WHO) strongly advocate the surveillance of the resistance of antibiotics.

Methods: During the study Clinical isolates (n=60) were collected from four different regions of Karachi. Antimicrobial susceptibility testing was performed by Kirby-Bauer disk diffusion method.

Results: Among the 291 clinical isolates evaluated, a large number of isolates were contributed by several Laboratories in various geographic areas of Karachi. 201 (69%) bacterial isolates were collected from Laboratory 1, 72 (25%) from Laboratory 2 and 18 (6%) from laboratory 3.

Among all (25) were Pseudomonas Specie & (35) were Pseudomonas aureginosa. Antibiotic susceptibility tests were performed for Pseudomonas aureginosa (n=35) & Pseudomonas specie (n=25) .Ciprofloxacin and Colistin were found to be the most effective antibiotics for P. aureginosa and for Pseudomonas specie colistin and polymixin B were the suitable options.

Conclusion: This study revealed that Pseudomonas was the predominant bacterial pathogen of soft tissue infection (41%), bacteraemia (24%) and nosocomial Urinary tract infection (17%) in Karachi Pakistan. It also demonstrate that due to era of resistance to number of extended spectrum beta-lactamase antibiotics, it is high time to enhanced surveillance for these organisms at unit-specific, institutional and national level. This study is valuable for Clinician in order to get better empiric treatment.