Advanced Study on Antioxidant Properties of Silver Nanoparticles Biosynthesized from Methanolic Leaf Extract of Blighia sapida

Synthesis of nanoparticles by biological methods using microorganisms, enzymes or plant extracts has been suggested as possible ecofriendly alternative to chemical and physical methods which involve the use of harmful reducing agents. In the current study, silver nanoparticles (AgNPs) were synthesized by green approach from methanolic leaf extract of Blighia sapida. The synthesized AgNPs were characterized by UV-visible (UV-vis) spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy and Scanning Electron Microscopy (SEM). The antioxidant activity was evaluated using DPPH radical scavenging assay, determination of total reductive potential, total phenolics content (TPC) and total flavonoids content (TFC) of the synthesized AgNPs. SEM analysis revealed that the size of the synthesized silver nanoparticles ranged from 50-70 nm with maximum UV-vis absorbance at 413 nm. DPPH radical scavenging activity, reducing power, total phenolic and total flavonoid contents of the synthesized AgNPs increased in a dose dependent manner as compared to ascorbic acid the standard reference used. This result confirmed that Blighia sapida is a potential biomaterial for synthesizing AgNPs which can be exploited for its antioxidant activity. This study provides an insight into the usage of Blighia sapida leaf as a good source of naturally occurring antioxidant and could have great importance as therapeutic agent in preventing or ameliorating oxidative stress related diseases.