Phylogenetic Detection of Patho Genic Escherichia coli Isolated from Different Sources in Najaf Hospitals

Najm, Rusul and Mohammed, Jinan (2023) Phylogenetic Detection of Patho Genic Escherichia coli Isolated from Different Sources in Najaf Hospitals. UTTAR PRADESH JOURNAL OF ZOOLOGY, 44 (5). pp. 55-59. ISSN 0256-971X

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Abstract

The Aim: The training intended to quarantine bacteria Escherichia coli from urinary tract infection, wound, burns patients and investigating the genes ChuA, yjaA, TspE4.C2 of the phylogenetic groups.

Study Design: 100 samples were collected from patients with urinary tract infection, wounds and burns, for a period from the twenty-seventh of July 2022 to the eleventh of December 2023, and after diagnosing the samples in the laboratory by means of selective media, the addition of the VITEK-2 compact.

Place and Duration of Study: All samples collected from July to December 2023. Samples were cultured on a MacConkey and Eosin Methylene Blue agar (EMB) and VITEK2 compact.

Methodology: A total of 100 sample from bacteria Escherichia coli sample Isolated from urine, wounds, burns Collected from several hospitals in city of Najaf included (Al-Sadr Teaching Hospital, Al-Najaf Teaching Hospital, Al-Furat Al-Awsat Hospital, Al-Hakim General Hospital, Al-Zahra Teaching Hospital, Burns Center, Public Health Laboratory).

Results: The results showed that the incidence of urinary tract infection 91%, and wound was 7%, while burns was 2%. the genes ChuA, yjaA, TspE4.C2 of the phylogenetic groups isolates were investigated using the PCR technique.

Conclusion: they were classified into four groups according to the presence or absence of these genes, so the results for group B1 was (44%), B2 (43%), D (12%), and A (1%).

Item Type: Article
Subjects: Library Keep > Biological Science
Depositing User: Unnamed user with email support@librarykeep.com
Date Deposited: 18 Oct 2023 10:21
Last Modified: 18 Oct 2023 10:21
URI: http://archive.jibiology.com/id/eprint/1633

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